UNcle All-in-One Biologics Stability Platform

UNcle combines 3 separate biologics instruments into a single package, saving you space, time and effort. UNcle uses three different measuring modes, i.e.

Fluorescence
SLS (Static Light Scattering)
DLS (Dynamic Light Scattering)

By incorporating all these modes into a single instrument you can generate a comprehensive analysis in just a few hours while at the same time using significantly less of your precious protein. This simplifies the process of narrowing down the optimal protein or formulation, resulting in faster research.

Range of Measurements

Use UNcle to determine the following properties of your proteins:

T_m & T_agg
Isothermal stability
Thermal recovery
Sizing
Polydispersity
Sizing with thermal ramp
k_D
B₂₂
Viscosity

Total Flexibility

UNcle allows you to generate more data with less sample. With just 9uL of sample required you can run as few or as many samples (up to 48) at a time, multiple times a day. You can run a DLS experiment and read it if thats all you need or alternatively, check DLS and then commence a 3 day experiment to monitor real-time stability.

Using specimens sealed in air-tight vessels you can perform experiments that are as short or long as you want.

Full Spectrum Analysis

Because biologics all behave differently, UNcle has been designed to provide you with the entire fluorescence spectrum. This means that you dont have to know ahead of time how your protein will behave. There is also no need to add dyes.

UNcle is able to detect aggregation on two wavelengths ensuring you dont miss either small or large aggregation.

One Shot, More Data

If you are experimenting with new formulations or constructs you can refine your results much more quickly. UNcle allows you to generate data on 48 samples in less than 2 hours. You can measure T_m and T_agg simultaneously and determine when unfolding leads to aggregation.

You can also generate DLS data before the temperature ramp and eliminate a sample straight away if it has aggregation issues.

Isothermal Stability

UNcle provides excellent thermal stability. This means you can perform SLS or DLS experiments over several days to determine long-term stability. All you need to do is program in the desired temperature and walk away.

Dynamic Light Scattering

Using the DLS function, UNcle allows you to quickly determine polydispersity, size distribution with amped sensitivity. Because UNcle is a multi-functional instrument you can then run other experiments such as a thermal ramp without even removing your sample.

B₂₂ and k_D

UNcle will also allow you to determine B₂₂ and k_D at the same time. This enables you to figure out immediately if your protein formulation combination has a good chance of success of if you should optimise further.