mirrorball – High Sensitivity Cytometer

mirrorball is a next generation laser-scanning microplate cytometer producing accurate, quantitative data for a wide range of applications, including fluorescent cell- and bead-based assays.

Key Benefits

Key benefits of the mirrorball cytometer include:

Homogeneous no-wash assays high sensitivity with no washing, allowing assays compatible with both adherent and non-adherent lines
Simple mix-and-read assay format eliminates the need for wash and incubation steps of ELISA. Simpler format also enables automation and reduced reagent requirements
High speed multiplexing first microplate cytometer to offer simultaneous laser scanning, speeding up assays by a factor of 3. This enables enhanced multiplexing and analytical capabilities and accelerated data generation
High sensitivity flow cytometry in a microplate an alternative to flow cytometry for the detection of low affinity antibodies and proteins found in low abundance
High content imaging whole well imaging makes statistically relevant results easier to achieve
Easy to use Cellista is a template-driven software package designed for multiple user environments

Applications

mirrorballs high sensitivity and superior multiplexing capabilities make it ideal for antibody discovery and a range of multiparametric high content imaging applications. In-well analysis ensures compatibility with adherent suspension cells, eliminating the upstream sample preparation of traditional flow cytometer or ELISA assays. mirroball offers new opportunities for highly sensitive detection in a wide range of assays. Typical applications include:

Monoclonal antibody development primary screening and lead optimization
High sensitivity cytometry detection and quantification of proteins and analytes present in low abundance e.g. intracellular kinases, GFP constructs, transporter proteins, cell surface receptors
Quantitation assays quantitate multiple analytes in wells using beads coated with capture protein. Suitable with beads of different fluorescent intensity